一种基于壳聚糖固定葡萄糖氧化酶的葡萄糖生物传感器的研究

本文选用生物相容性好的壳聚糖作为基体材料，使其与戊二醛交联成网状结构包埋葡萄糖氧化酶制成电化学传感器。这种壳聚糖膜不仅可以减小葡萄糖氧化酶的流失，而且能为酶提供了适宜的微环境。用红外光谱、紫外光谱及透射电镜对膜的形态和性质进行了表征。实验结果表明该传感器具有很快的响应速度，很好的稳定性和重现性，能选择性地催化葡萄糖并测定其浓度。该传感器的制备方法简单，成本低，于冰箱中放置两周信号保持在90％以上，对葡萄糖测量的线性范围为1×10^-5 - 3.4×10^-3mol•L^-1，当信噪比为3:1时检测限为5×10^-6mol•L^-1。

A Glucose Biosensor Based on Immobilization of Glucose Oxidase in Chitosan Network Matrix

A simple and promising glucose biosensor was constructed by successful entrapment of glucose oxidase (GOD) into chitosan matrix, which was cross‐linked with glutaraldehyde (GA). This material provided good biocompatibility and the stabilizing microenvironment around the enzyme. The morphologies and properties of chitosan and chitosan/GOD were characterized by FTIR, UV‐Vis and SEM techniques. This biosensor exhibited a fast amperometric response to glucose. The linear range for glucose determination was from 1×10^?5 to 3.4×10^?3 mol·L^?1, with a detection limit of 5×10^?6 mol·L^?1 based on S/N=3. The biosensor could retaCin ca. 90% of its original activity after two weeks of storage under dry conditions at 4 °C.