Modulation of hemocompatibility of polysulfone by polyelectrolyte multilayer films |
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| Affiliation: | 1. Department of Chemical Engineering, National Taiwan University, No. 1, Roosevelt Rd., Sec. 4, Taipei 106, Taiwan;2. Department of Chemical Engineering, National Taiwan University of Science and Technology, Taipei 106, Taiwan;3. R&D Center for Membrane Technology and Department of Chemical Engineering, Chung Yuan Christian University, Chungli, Taoyuan, Taiwan;1. School of Chemistry and Chemical Engineering, Shihezi University, Shihezi 832003, China;2. Engineering Research Center of Materials-Oriented Chemical Engineering of Xinjiang Production and Construction Corps, Shihezi 832003, China;3. Key Laboratory of Materials-Oriented Chemical Engineering of Xinjiang Uygur Autonomous Region, Shihezi 832003, China;1. School of Physics and Electronic Science, Fuyang Normal College, Fuyang 236037, China;2. Changchun Institute of Optics, Fine Mechanics and Physics, Chinese Academy of Sciences, Changchun 130033, China;3. Department of Physics, College of Science, Qiqihar University, Qiqihar 161006, China;4. Jilin Provincial Key Laboratory of Applied Atomic and Molecular Spectroscopy, Institute of Atomic and Molecular Physics, Jilin University, Changchun 130012, China;1. School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang 212013, China;2. State Key Laboratory of Coordination Chemistry, Nanjing University, Nanjing 210093, China;1. Department of Materials Science and Engineering and Chemical Engineering, University Carlos III of Madrid, 28911 Leganés, Madrid, Spain;2. LEPMI UMR 5279 CNRS-Grenoble-INP-UdS-UJF, Bât. PHELMA, BP.75 Fr., 38402 St. Martin d’Hères Cedex, France |
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| Abstract: | Polyelectrolyte multilayer (PEM) films have been recently applied to surface modification of biomaterials. Cellular interactions with PEM films consisted of weak polyelectrolytes are greatly affected by the conditions of polyelectrolyte deposition, such as pH of polyelectrolyte solution. Previous studies indicated that the adhesion of several types of mammalian cells to PAH/PAA multilayer films was hindered by low pH and high layer numbers. The objective of this study is to evaluate whether the hemocompatibility of polysulfone can be modulated by deposition of poly(allylamine hydrochloride) (PAH)/poly(acrylic acid) (PAA) multilayer films. PAH/PAA multilayer films with different layer numbers were assembled onto polysulfone at either pH 2.0 or pH 6.5. The number of platelet adhesion and the morphology of adherent platelets were determined to evaluate hemocompatibility of modified substrates. Compared to non-treat polysulfone, the PEM films developed at pH 2.0 decreased platelet adhesion, while those built at pH 6.5 enhanced platelet deposition. Platelet adhesion was found positively correlated to polyclonal antibodies binding to surface-bound fibrinogen. The extent of platelet spreading was increased with layer numbers of PEM films, suggesting that the adherent platelets on thick PEM films were prone to activation. In conclusion, PAH/PAA films with few layers developed at pH 2.0 possessed better hemocompatibility compared to other substrates. |
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