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Determination of the Anticancer Drug Sorafenib in Serum by Adsorptive Stripping Differential Pulse Voltammetry Using a Chitosan/Multiwall Carbon Nanotube Modified Glassy Carbon Electrode |
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| Authors: | Nurgul Karadas‐Bakirhan Stéphanie Patris Sibel A Ozkan Alp Can Jean‐Michel Kauffmann |
| Institution: | 1. Ankara University, Faculty of Pharmacy, Department of Analytical Chemistry, 06100 Tandogan, Ankara, Turkey;2. Université libre de Bruxelles (ULB), Faculty of Pharmacy, Laboratory of Instrumental Analysis and Bioelectrochemistry, Campus Plaine, CP 205/6, 1050 Bruxelles, Belgium;3. Ankara University, Faculty of Medicine, Department of Histology and Embryology, 06410, Sihhiye Ankara, Turkey |
| Abstract: | Adsorptive stripping differential pulse voltammetry (AdSDPV) was applied to the assay of sorafenib in human serum sample. Cyclic voltammetry at a carbon based screen printed electrode (SPE) permitted to detect the irreversible oxidation of SOR with formation of a new compound reversibly oxidized at a lower potential. Quantitative assays were realized using a chitosan/carboxylic acid functionalized multiwalled carbon nanotube modified glassy carbon electrode in 0.1 M phosphate buffer pH 7.0 in the presence of 50 % methanol. The AdSDPV method provided two linear responses within the concentration ranges 1×10?8–8×10?8 M and 1×10?7–8×10?7 M in serum with LOQ and LOD of 3.2×10?9 and 9.6×10?10 of lower linear range, respectively. The recovery of sorafenib in spiked serum was 97.5 %. |
| Keywords: | Anticancer Drug Sorafenib Adsorptive Stripping Differential Pulse Voltammetry Chitosan |