Electrochemical properties of niosomes modified Au electrode and DNA recognition |
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| Institution: | 1. Departament d′Enginyeria Quıímica, Universitat Rovira i Virgili, Avinguda Països Catalans 26, 43007 Tarragona, Spain;2. Sorbonne Université, Institut Parisien de Chimie Moléculaire, UMR CNRS 8232, 4 place Jussieu, 75005 Paris, France;3. ICREA, Passeig Lluis Companys 23, 08010 Barcelona, Spain;1. State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Changchun, Jilin 130022, China;2. University of Science and Technology of China, Hefei, Anhui 230026, China;3. University of Chinese Academy of Sciences, Beijing 100039, China;1. Shandong Provincial Key Laboratory of Biochemical Engineering, Qingdao Nucleic Acid Rapid Detection Engineering Research Center, College of Marine Science and Biological Engineering, Qingdao University of Science and Technology, Qingdao, 266042, Shandong, China;2. Qingdao Nucleic Acid Rapid Testing International Science and Technology Cooperation Base, College of Life Sciences, Department of Pathogenic Biology, School of Basic Medicine, and Department of Clinical Laboratory, The Affiliated Hospital of Qingdao University, Qingdao University, Qingdao, 266071, Shandong, China;3. Department of Biomedical Sciences, City University of Hong Kong, Hong Kong, 999077, PR China;1. State Key Laboratory of Environmental Friendly Energy Materials, School of Materials Science and Engineering, Southwest University of Science and Technology, Sichuan 621010, China;2. School of Civil Engineering and Architecture, Southwest University of Science and Technology, Sichuan 621010, China;3. School of Materials Science and Engineering, Southeast University, Nanjing, Jiangsu 211189, China;1. Department of Chemical Sciences and Technologies, University of Rome Tor Vergata, Via della Ricerca Scientifica 1, 00133 Roma, Italy;2. Department of Electrical and Information Engineering, University of Cassino and Southern Lazio, 03043 Cassino FR, Italy;3. S2G Technologies Srl Tecnopolo Tiburtino, Via Giacomo Peroni 444, 00131 Roma, Italy;4. INFN-Laboratori Nazionali di Frascati, Via E. Fermi 54, 00044 Frascati, Italy;5. CREA Research Centre for Olive, Fruit and Citrus Crops, Via di Fioranello 52, 00134 Rome, Italy;6. Istituto di Farmacologia Traslazionale-CNR (IFT-CNR), Via Fosso del Cavaliere 100, 00133 Roma, Italy;7. Istituto di Struttura della Materia-CNR (ISM-CNR), Via Fosso del Cavaliere 100, 00133 Roma, Italy;8. Department of Physics, University of Rome Tor Vergata, Via della Ricerca Scientifica 1, 00133 Roma, Italy;1. Department of Chemistry, Rhodes University, P.O. Box 94, Grahamstown, 6140, South Africa;2. Institute for Nanotechnology Innovation, Department of Chemistry, Rhodes University, P.O. Box 94, Grahamstown, 6140, South Africa |
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| Abstract: | Non-ionic surfactant vesicles (NSVs), also referred to as niosomes, have been studied as an alternative to conventional liposomes. In this paper, electrochemical inspection of the interaction between Herring sperm DNA and niosomes has been investigated after a simple and novel method for the formation of niosomes on Au electrode. Each step of electrode modification has been confirmed with cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The assembly of octadecanethiol (ODT) layer on the electrode surface generates a packed film that introduces a barrier to the interfacial electron transfer (Ret), and the subsequent immobilization of niosomes onto the self-assembled monolayer (SAM) layer results in a further increase of Ret, due to the formed bilayer almost blocked the redox probe to the electrode surface. When Herring sperm DNA was added, the Ret value decreased, indicating that the barrier of the redox probe to the surface was disrupted. The addition of DNA caused the formation of some transmembrane channels for the redox probe across the niosomes. A good linear relationship between Ret value and DNA concentration was found over the 0–0.05 mg mL−1 concentration range. |
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