| 聚二甲基硅氧烷芯片自由酶反应器检测葡萄糖 |
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| 引用本文： | 仲海燕,周洁,余晓冬,陈洪渊.聚二甲基硅氧烷芯片自由酶反应器检测葡萄糖[J].分析化学,2010,38(6). |
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| 作者姓名： | 仲海燕 周洁 余晓冬 陈洪渊 |
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| 作者单位： | 南京大学化学化工学院,生命分析化学教育部重点实验室,南京,210093 |
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| 基金项目： | 国家自然科学基金创新群体新项目，973项目，863项目 |
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| 摘 要： | 应用电泳中介微分析(EMMA)技术,构建聚二甲基硅氧烷(PDMS)芯片自由酶反应器, 在线检测葡萄糖(Glu),在十字形的芯片通道上,采用自制的碳纤维微电极检测葡萄糖氧化酶(GOD)催化氧化Glu生成的H2O2,并对检测电位、GOD浓度、GOD进样时间、分离电压等参数进行了优化,测定了该自由酶反应器的线性范围和检出限,考察了其重现性及稳定性.结果表明,此自由酶反应器制作方便,操作简单,重现性好,Glu浓度在0.1～20 mmol/L之间有较好的线性关系(r=0.997),检出限为19.8 μmol/L(S/N=3).
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| 关 键 词： | 自由酶反应器 葡萄糖 电泳中介微分析 聚二甲基硅氧烷芯片 |
Detection of Glucose on Free Enzyme-based Poly (dimethylsiloxane) Microchip |
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| ZHONG Hai-Yan,ZHOU Jie,YU Xiao-Dong,CHEN Hong-Yuan.Detection of Glucose on Free Enzyme-based Poly (dimethylsiloxane) Microchip[J].Chinese Journal of Analytical Chemistry,2010,38(6). |
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| Authors: | ZHONG Hai-Yan ZHOU Jie YU Xiao-Dong CHEN Hong-Yuan |
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| Institution: | ZHONG Hai-Yan,ZHOU Jie,YU Xiao-Dong,CHEN Hong-Yuan (The Key Lab of Analytical Chemistry for Life Science,Ministry of Education,School of Chemistry and Chemical Engineering,Nanjing University,Nanjing 210093) |
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| Abstract: | Microfluidic enzyme-based reactor could be used to detect the biomolecules with high sensitivity and selectivity. Based on the electrophoretic mediated microanalysis (EMMA) method, a new kind of free enzyme-based poly(dimethylsiloxane) (PDMS) microchip was developed to detect glucose (Glu). On-line catalysis reaction of Glu by glucose oxidase (GOD) was carried out on the chip. The product H2O2 was detected using single carbon fibre cylindrical electrode. Factors influencing the separation and detection, suc... |
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| Keywords: | Free enzyme-based microchip Glucose Electrophoretic mediated microanalysis Poly(dimethylsiloxane) |
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