半导体纳米粒子与金纳米粒子间荧光共振能量转移研究

采用水相法合成的CdTe半导体纳米粒子作为能量给体, 通过Schiff碱反应将单链DNA连接到表面. 采用柠檬酸钠还原氯金酸法制取的Au纳米粒子作为能量受体, 通过Au—S键将单链DNA连接到表面. 通过DNA链间的杂交, 构建了荧光共振能量转移体系(FRET). 测定了CdTe-DNA、 探针体系和探针体系+目标DNA的荧光强度. 结果表明, 探针体系的荧光强度最弱, 加入目标DNA后, 体系荧光增强, 表明该体系的构建是成功的.

Fluorescence Resonance Energy Transfer Between CdTe Quantum Dot Donors and Au Nanoparticles Labeled DNA Acceptors

CdTe semiconductor nanoparticles which were synthesized in water phase were taken as the energy donors. CdTe nanoparticles were linked to 5'-NH2-DNA by Schiff's reaction. Au nanoparticles which were prepared by sodium citrate reduction of chloroauric acids were taken as the energy acceptors. Au nanoparticles were linked to 3'-SH—DNA by Au—S bond. The fluoresscence resonance energy transfer(FRET) probes are generated through hybridization of complementary DNA bound to the QDs and Au nanoparticles. The fluorescence spectra of CdTe-DNA, CdTe-DNA-Au and CdTe-DNA-Au+target DNA were measured . The data show that the fluorescence intensity of CdTe-DNA-Au conjugates was the weakest. When adding a complementary ssDNA to CdTe-DNA-Au conjugates, the fluorescence intensity of the system increased. This result shows that the configuration of FRET was successful.