亲水金和憎水二氧化硅纳米颗粒对葡萄糖生物传感器响应灵敏度的增强作用

用亲水金、憎水二氧化硅纳米颗粒固定葡萄糖氧化酶(GOD),采用聚乙烯醇缩丁醛(PVB)为辅助固酶膜基质来制备葡萄糖生物传感器,并考察了亲水金、憎水二氧化硅纳米颗粒对酶电极电流响应的影响.实验表明,引入纳米粒子可显著增强电极响应灵敏度.并对两种不同性质纳米颗粒所起作用的可能机理进行讨论,从理论和实验上证明了纳米颗粒对固定酶的作用.为制备有实用价值的葡萄糖生物传感器提供了可供参考的实验和理论依据.

Enhancement Effect of Hydrophilic Au and Hydrophobic SiO2 Nanoparticles on Glucose Biosensor Sensitivity

The construction of glucose biosensor by immobilization of glucose oxidase(GOD) on the surface of hydrophilic Au and hydrophobic SiO_2 nanoparticles is reported, and polyvinyl butyral(PVB) is used as auxiliary immobilization enzyme matrix. The data of the current response of enzyme electrodes containing hydrophilic Au and hydrophobic SiO_2 nanoparticles to glucose are obtained. The experiments show that the nanoparticle would have a very strong enhancement effect on the GOD electrode response sensitivity. A mechanism for the behavior of the different kind of nanoparticles is proposed. The experiment and theory proved the function of nanoparticles in immobilization enzyme. This report provides experimental and theoretical basis for preparing the useful glucose biosensor.