Transsialidase from Trypanosoma cruzi for regio- and stereoselective synthesis of N-acyl-modified sialylated oligosaccharides and measurement of transfer rates |
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| Authors: | Schroven Andreas Meinke Sebastian Ziegelmüller Patrick Thiem Joachim |
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| Affiliation: | University of Hamburg, Faculty of Science, Department of Chemistry, Institute of Organic Chemistry, Martin-Luther-King Platz 6, 20146, Hamburg, Germany. |
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| Abstract: | ![]()
Recombinant transsialidase from Trypanosoma cruzi (TcTS) was used for the sialylation with natural and non-natural derivatives of neuraminic acid. Neu5Ac-alpha(2-->3)-Gal-beta(1-->6)-Glc-alphaOMe was prepared in 80 % yield. Correspondingly, the modified trisaccharide derivatives Neu5Prop-alpha(2-->3)-Gal-beta(1-->6)-Glc-alphaOMe (32 %) and Neu5Gc-alpha(2-->3)-Gal-beta(1-->6)-Glc-alphaOMe (Prop=propanoyl, Gc=glycolyl) were obtained in 60 % yield, respectively. |
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| Keywords: | enzyme catalysis glycosylation neuraminic acid sialylation transsialidase |
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