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| 基于电化学聚合固定抗体的电化学发光免疫分析法检测人免疫球蛋白 | |
| 引用本文： | 云雯,王晓英,董平,朱金坤,徐颖,何品刚,方禹之. 基于电化学聚合固定抗体的电化学发光免疫分析法检测人免疫球蛋白[J]. 分析化学, 2009, 37(1) |
| 作者姓名： | 云雯 王晓英 董平 朱金坤 徐颖 何品刚 方禹之 |
| 作者单位： | 华东师范大学化学系,上海,200062 |
| 基金项目： | 国家自然科学基金，上海市科学技术委员会资助项目 |
| 摘 要： | 基于电化学聚合在金电极表面固定兔抗人免疫球蛋白G抗体与人免疫球蛋白G及标记有Ru(bpy)2+3 的羊抗人免疫球蛋白G抗体之间发生特异性免疫反应,形成三明治结构,成功建立了用于测定人血清中免疫球蛋白G的电化学发光(ECL)免疫技术.利用此方法测定人免疫球蛋白G含量,浓度在50 μg/L～2 mg/L范围内与电化学发光强度呈良好的线性关系,线性回归方程为y(a. u.)=48.41+0.09x(μg/L) (n=7);检出限为20 μg/L (3σ).测得正常人血清中免疫球蛋白G平均含量为11.2 g/L ,结果令人满意. |
| 关 键 词： | 人免疫球蛋白G 电化学发光 免疫检测 电化学聚合 |
Electrogenerated Chemiluminescence Immunoassay for Human IgG with Electrochemical Polymerization-based Immobilization Method |
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| YUN Wen,WANG Xiao-Ying,DONG Ping,ZHU Jin-Kun,Xu Ying,He Pin-Gang,Fang Yu-Zhi. Electrogenerated Chemiluminescence Immunoassay for Human IgG with Electrochemical Polymerization-based Immobilization Method[J]. Chinese Journal of Analytical Chemistry, 2009, 37(1) | |
| Authors: | YUN Wen WANG Xiao-Ying DONG Ping ZHU Jin-Kun Xu Ying He Pin-Gang Fang Yu-Zhi |
| Affiliation: | Department of Chemistry;East China Normal University;Shanghai 200062 |
| Abstract: | Rabbit-anti-human IgG was immobilized on gold electrode by electrochemical copolymerization with pyrrole.An efficient immunoassay strategy for the determination of human IgG in human serum was developed based on the specific immunological reaction between the rabbit-anti-human IgG,human IgG and Ru(bpy)2+3-labled goat-anti-human IgG,which formed sandwich-typed immunocomplex.Then the target IgG was determined by electrogenerated chemiluminescence(ECL).The parameters of polymerization time,incubation time and ... |
| Keywords: | Human immunoglobulin G electrochemiluminescence immunoassay electrochemical polymerization |
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