| 聚丙烯酸甲酯载体对柱状假丝酵母脂肪酶固定化的研究（Ⅰ） |
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| 引用本文： | 徐慧显,李民勤.聚丙烯酸甲酯载体对柱状假丝酵母脂肪酶固定化的研究（Ⅰ）[J].离子交换与吸附,1994,10(4):316-321. |
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| 作者姓名： | 徐慧显 李民勤 |
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| 作者单位： | 南开大学高分子化学研究所 |
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| 摘 要： | 合成了一系列不同结构的聚丙烯酸甲酯，考察了它们的固定化酵母脂肪酶催化酯水解反应的效果，得到了载体孔结构对固定化效果影响的一些规律．研究了最佳吸附条件，比较ｐＨ和离子强度对酵母脂肪酶自由酶和固定化酶催化酯水解反应活力的影响．
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| 关 键 词： | 柱状假丝酵母脂肪酶，固定化酶，聚丙烯酸甲酯，酯水解反应 |
Studies on Immobilization of Candida Cylindracea Lipase on Poly(methyl acrylate)Carriers |
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| Xu Huixian,Li Minqin,Wang Daobin,He Binglin.Studies on Immobilization of Candida Cylindracea Lipase on Poly(methyl acrylate)Carriers[J].Ion Exchange and Adsorption,1994,10(4):316-321. |
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| Authors: | Xu Huixian Li Minqin Wang Daobin He Binglin |
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| Abstract: | A series of poly(methyl acrylate)s of different structure were synthesized. The activity of Candida cylindracea lipase immobilized on these carriers was studied in catalyzing hydrolysis of ester.Some regulations of the activity of immobilized lipase influencex by the pore structure of the cariers wee found.The optimal conditions adsorbing lipase were studied.The effects of pH and ionic strength in the free lipase were compared with those on the immobilized lipase in catalyzing hydrolysis of ester. |
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| Keywords: | Candida cylindracea Immobilized enzyme Poly(methyl acrylate) Hydrolysis of ester |
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