Effect of fatty acid methyl esters from plastrum testudinis on proliferation of rat bone mesenchymal stem cells |
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| Authors: | Yuhua Zhang Heping Zeng Dongfeng Chen |
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| Institution: | (1) Department of Biological and Environmental Engineering, Guangzhou City Polytechnic, Guangzhou, 510405, China;(2) Institute of the Functional Molecule, Department of Chemistry, South China University of Technology, Guangzhou, 510641, China;(3) Guangzhou University of Traditional Chinese Medicine, Guangzhou, 510405, China |
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| Abstract: | The ointment of plastrum testudinis was extracted using petroleum ether, ether and dichloromethane sequentially and the extracts
were methyl-esterified. The effects on the proliferation of bone marrow mesenchymal stem cells (bMMSCs) were examined by MTT3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl
tetrazolium bromide] assay and flow cytometry analysis. The volatile components of the samples were studied by gas chromatography-mass
spectrometry (GC-MS) and high performance liquid chromatography (HPLC). The results show that the methyl-esterified parts
can promote the proliferation of stem cells and they all contain palmitic acid methyl ester. Palmitic acid methyl ester can
promote proliferation when the concentration was 0.15 μg/μL. It may be concluded that the palmitic acid methyl ester is important
for the methyl-esterified parts that have effects on proliferation.
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Translated from Chinese Journal of Analytical Chemistry, 2007, 35 (10): 1400–1404 译自: 分析化学] |
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| Keywords: | plastrum testudinis stem cell proliferation fatty acid methyl esterify |
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