| 分子印迹柱-高效液相色谱法测定猪肉中磺胺嘧啶(英文) |
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| 引用本文： | 黄运红,许杨,杜碧柏,何庆华,曹郁生. 分子印迹柱-高效液相色谱法测定猪肉中磺胺嘧啶(英文)[J]. 分析化学, 2012, 0(7): 1011-1018 |
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| 作者姓名： | 黄运红 许杨 杜碧柏 何庆华 曹郁生 |
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| 作者单位： | 食品科学与技术国家重点实验室南昌大学中德联合研究院;江西师范大学生命科学学院 |
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| 基金项目： | supported by the Hi-Tech Research and Development Program(863)of China(No.2007AA10Z427);the National Natural Science Foundation of China(No.30860123);the Research Program of State Key Laboratory of Food Science and Technolo-gy,Nanchang University(No.SKLF-MB-201002) |
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| 摘 要： | 建立了分子印迹柱-高效液相色谱法检测猪肉中磺胺嘧啶残留方法。制备了磺胺嘧啶的分子印迹柱,并优化了分子印迹柱的萃取条件。研究结果表明,在最佳萃取条件下,分子印迹柱-高效液相色谱法的加标回收率≥75.6%,相对标准偏差≤6.1%。分子印迹柱为固相萃取柱可以预浓缩与纯化猪肉样品中磺胺嘧啶。与氧化铝萃取柱比,分子印迹柱具有较好的重复性和萃取效率。本方法已成功用于实际猪肉样品中磺胺嘧啶含量的检测,结果满意。
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| 关 键 词： | 分子印迹柱 高效液相色谱 磺胺嘧啶 猪肉 |
Determination of Sulfadiazine Residues in Pork by Molecular Imprinted Column Coupling with High Performance Liquid Chromatography |
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| HUANG Yun-Hong,XU Yang,DU Bi-Bai,HE Qing-Hua,CAO Yu-Sheng. Determination of Sulfadiazine Residues in Pork by Molecular Imprinted Column Coupling with High Performance Liquid Chromatography[J]. Chinese Journal of Analytical Chemistry, 2012, 0(7): 1011-1018 |
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| Authors: | HUANG Yun-Hong XU Yang DU Bi-Bai HE Qing-Hua CAO Yu-Sheng |
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| Affiliation: | 1(State Key Laboratory of Food Science and Technology,Sino-Germany Joint Research Institute, Nanchang University,Nanchang 330047,China) 2(College of Life Science,Jiangxi Normal University,Nanchang 330022,China) |
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| Abstract: | A method for the quantitative monitoring of sulfadiazine(SD) residues in pork was established by molecular imprinted column coupling with high performance liquid chromatography(HPLC).The molecular imprinted column was selected as an extraction device.To obtain the optimum extraction efficiency,several parameters related to the molecular imprinted column,including column solvent,flow-rate,eluent of the sample matrix and eluent volume,were investigated.The sample solution was directly injected into the device for the extraction after simple extraction.Under the optimum conditions,the relative standard deviations(RSD) was ≤6.1% and the recoveries for SD were higher than 75.6%.In comparison with the AL-SPE column,the MIP-SPE column had good reusability and extraction efficiency.This method was successfully applied to the determination sulfadiazine residues in pork. |
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| Keywords: | Molecular imprinted column High Performance Liquid Chromatography Sulfadiazine Pork |
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