| 纳米金标记-膜过滤分光光度法检测免疫球蛋白G |
| |
| 引用本文： | 梁爱惠,黄文昭,潘杰斌,谭玉前,金文英.纳米金标记-膜过滤分光光度法检测免疫球蛋白G[J].分析试验室,2010,29(1). |
| |
| 作者姓名： | 梁爱惠 黄文昭 潘杰斌 谭玉前 金文英 |
| |
| 作者单位： | 桂林理工大学化学与生物工程学院,桂林,541004 |
| |
| 基金项目： | 国家自然基金(20965002);;广西自然基金(桂科自0832260);;广西教育厅基金(200707LX202)项目资助 |
| |
| 摘 要： | 采用10 nm的纳米金标记羊抗人免疫球蛋白G获得免疫球蛋白G(IgG)的探针(AuIgG)。在pH 6.8的NaH2PO4-Na2HPO4磷酸盐缓冲溶液及聚乙二醇6000、KCl溶液存在下,IgG与AuIgG探针发生免疫反应,用0.15μm滤膜过滤反应生成的免疫复合物溶液,滤液在524 nm处有一最大吸收峰。其降低值ΔA524 nm随着IgG浓度的增加线性增加,据此建立了一种测定IgG的分光光度法。在最佳实验条件下,免疫球蛋白G浓度在0.025~0.375μg/mL范围内与ΔA呈良好的线性关系,其线性回归方程为ΔA=0.783ρ+0.0232,相关系数为0.9927,检出限(3σ)为0.0082μg/mL。该法用于分析人血清中免疫球蛋白G,结果与免疫透射比浊法结果一致,相对标准偏差在2.0%~5.6%之间。
|
| 关 键 词： | 免疫球蛋白G 纳米金标记 膜过滤 分光光度法 |
Immunonanogold-labeled spectrophotometric determination of trace IgG based on membrane filtration |
| |
| LIANG Ai-hui,HUANG Wen-zhao,PAN Jie-bin,TAN Yu-qian,JIN Wen-ying.Immunonanogold-labeled spectrophotometric determination of trace IgG based on membrane filtration[J].Chinese Journal of Analysis Laboratory,2010,29(1). |
| |
| Authors: | LIANG Ai-hui HUANG Wen-zhao PAN Jie-bin TAN Yu-qian JIN Wen-ying |
| |
| Institution: | School of Chemical and Bioengineering;Guilin University of Technology;Guilin 541004 |
| |
| Abstract: | Nanogold particles of 10 nm were used to label goat anti-human IgG(GIgG) to obtain nanogold-labeled IgG(AuIgG).In the pH 6.8 NaH2PO4-Na2HPO4 buffer solution and in the presence of polyethylene glycol(PEG) 6000 and KCl,AuGIgG combined with IgG and aggregated to form immunocomplex AuIgG-IgG particles by Val der Waals force,hydrophobic force,coulomb attracting force and hydrogen bond binding force,and nanogold particles form larger nanogold clusters that can be removed by membrane filtration.With addition of I... |
| |
| Keywords: | Immunoglobulin G Nanogold-labeled Membrane filtration Spectrophotometry |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
