Development of a real-time capacitive biosensor for cyclic cyanotoxic peptides based on Adda-specific antibodies |
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Authors: | Lesedi Lebogang Martin Hedström Bo Mattiasson |
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Institution: | 1. Department of Biotechnology, Lund University, Box 124, 22100 Lund, Sweden;2. CapSenze HB, Annersbergs gård 5520, 26021 Billeberga, Sweden |
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Abstract: | The harmful effects of cyanotoxins in surface waters have led to increasing demands for accurate early warning methods. This study proposes a capacitive immunosensor for broad-spectrum detection of the group of toxic cyclic peptides called microcystins (∼80 congeners) at very low concentration levels. The novel analytical platform offers significant advances compared to the existing methods. Monoclonal antibodies (mAbs, clone AD4G2) that recognize a common element of microcystins were used to construct the biosensing layer. Initially, a stable insulating anchor layer for the mAbs was made by electropolymerization of tyramine onto a gold electrode surface, with subsequent incorporation of gold nanoparticles (AuNPs) on the glutaraldehyde (5%) activated polytyramine surface. The biosensor responded linearly to microcystin concentrations from 1 × 10−13 M to 1 × 10−10 M MC-LR standard with a limit of detection of 2.1 × 10−14 M. The stability of the biosensor was evaluated by repeated measurements of the antigen and by determining the capacitance change relative to the original response, which decreased below 90% after the 30th cycle. |
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Keywords: | Cyclic cyanotoxins Capacitive Adda-specific antibodies Electropolymerization Gold nanoparticles |
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