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| 氨基酸衍生物对映体的高效液相色谱手性分离 | |
| 引用本文： | 李海燕,梁鑫淼. 氨基酸衍生物对映体的高效液相色谱手性分离[J]. 分析试验室, 2005, 24(10): 5-7 |
| 作者姓名： | 李海燕 梁鑫淼 |
| 作者单位： | 中国科学院大连化学物理研究所,大连,116012;公安部物证鉴定中心,北京,100038;中国科学院大连化学物理研究所,大连,116012 |
| 摘 要： | 通过在流动相中使用酸性添加剂,在由(S)-N-(2-萘基)丙氨酸衍生而成的手性固定相上直接分离氨基酸的3,5-二硝基苯甲酰衍生物,获得非常理想的分离效果。并在此工作的基础上对手性识别机理进行了初步探讨。另外,通过在不同构型的手性固定相上分离相同的溶质,证明在结构相同、构型相反的手性固定相上,对映体的出峰顺序是相反的。 |
| 关 键 词： | 氨基酸衍生物 手性固定相 对映体分离 酸性添加剂 |
| 文章编号： | 1000-0720（2005）10-0005-03 |
| 收稿时间： | 2004-11-10 |
| 修稿时间： | 2005-01-10 |
Separation of Alanine Enantiomer Derivatives by Chiral HPLC |
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| LI Hai-yan,LIANG Xin-miao. Separation of Alanine Enantiomer Derivatives by Chiral HPLC[J]. Chinese Journal of Analysis Laboratory, 2005, 24(10): 5-7 | |
| Authors: | LI Hai-yan LIANG Xin-miao |
| Affiliation: | 1 Dalian Institute Of Chemical Physics, Chinese Academy of Sciences, Dalian 116012;2 Instimte of Fo- rensic Science, Ministry of Public Security, Beijing 100038 |
| Abstract: | The enantiomers of N-3,5-dinitrobenzoyl(DNB) of amino acid derivatives were resolved completely on a (S)-N-(2-naphthyl)-alanine derived chiral stationary phase(CSP) by adding a small amount of acetic acid in the mobile phase. And better peak shapes have been achieved. Then the chiral recognition mechanism was discussed. In addition, the separations of the enantiomers of the same analytes on their enatiomeric CSP were studied. It was indicated that the two columns of opposite chirality gave reversed elution orders. |
| Keywords: | Amino acid derivatives Chiral stationary phase Enantiomeric seperation Acidic additive |
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