Analysis of amino acid neurotransmitters by capillary electrophoresis and laser-induced fluorescence using a new fluorescein-derived label |
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Authors: | Liwei Cao Huashan Zhang Wang Hong |
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Institution: | (1) Department of Chemistry, Jinan University, 510632 Guangzhou, China;(2) Department of Chemistry, Wuhan University, 430072 Wuhan, China |
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Abstract: | A capillary electrophoresis laser-induced fluorescence detection method (CE-LIF) was developed for the separation of eight
neurotransmitters tagged on their amino function with 6-oxy-(N-succinimidyl acetate)-9-(2′-methoxycarbonyl) fluorescein (SAMF), a new fluorescent reagent synthesized in our lab. Derivatization
was performed in boric acid buffer (pH = 7.75) at 37 °C over 15 min. The pH-independent fluorescence of SAMF (pH 4–9) permits
background buffers over a wide range of pH. It was demonstrated that an acidic running buffer offers a better resolution compared
to basic medium in terms of resolution and peak shapes. Employing Cu2+ as the additive, the molecules were baseline-separated using a running buffer consisting of 40 mM sodium acetate and 2 mM
Cu2+ (pH 6.0). The detection limits ranged from 1 to 2 × 10−10 M. The method has been validated for the characterization of lymphocyte samples. The results obtained illustrate the advantages
of combining SAMF derivatization with CE-LIF for determining neurotransmitters. |
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Keywords: | : Fluorescent label amino acid neurotransmitters capillary electrophoresis laser-induced fluorescence lymphocyte |
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