| 苯酚、马尿酸和甲基马尿酸的反相高效液相色谱分析 |
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| 引用本文： | 郭伟强,杨国梁,汤淼荣,殷小晴,罗晓雯. 苯酚、马尿酸和甲基马尿酸的反相高效液相色谱分析[J]. 分析化学, 1995, 23(4): 449-452 |
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| 作者姓名： | 郭伟强 杨国梁 汤淼荣 殷小晴 罗晓雯 |
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| 作者单位： | 杭州大学化学系!杭州310028 |
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| 摘 要： | 本文提出了人体内苯、甲苯和二甲苯的代谢产物苯酚、马尿酸和甲基马尿酸的反相高效液相色谱分析法,讨论了其保留机制和样品预处理技术.以ODS为固定相,甲醇-水-醋酸为流动相时可实现良好分离.方法回收率为97.3%,相对标准偏差为1.02%.提出的方法可用于尿样分析,适用于临床与职业病防治的监测分析.
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| 关 键 词： | 反相高效液相色谱 马尿酸 预处理小柱 三元流动相 |
Reversed-phase High-performance Liquid Chromatographic Analysis of Phenol, Hippuric Acid and Metylhippuric Acid |
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| Guo Weiqiang,Yang Guoliang,Tang Miaorong,Yin Xiaoqing,Luo Xiaowen. Reversed-phase High-performance Liquid Chromatographic Analysis of Phenol, Hippuric Acid and Metylhippuric Acid[J]. Chinese Journal of Analytical Chemistry, 1995, 23(4): 449-452 |
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| Authors: | Guo Weiqiang Yang Guoliang Tang Miaorong Yin Xiaoqing Luo Xiaowen |
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| Abstract: | A determining method and retention mechanism ot phenol, hippuric acid and methylhippuric acid, metabolized from benzene, toluene and xylene in human body, have been reported. These three compounds can be separated well by RP-HPLC on ODS column and with methanol-water-acetic acid (32. 5 : 67. 07 : 0. 43) as mobile phase and UV detector at 254 nm. The recovery is 97. 3% ,and the relative standard deviation is 1. 02% , and the detection limit is 0. 54 ng. The method can be applied to the analysis of urine from persons exposed to benzene, toluene and xylene. |
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| Keywords: | Reversed-phase high-performance liquid chromatography hippuric acid pre-treatment column ternary mobile phase |
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