| Analysis of 17β-Estadiol from Sewage in Coastal Marine by Surface Plasmon Resonance Technique |
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| 作者姓名： | ZHANG Wei-wei CHEN Yu-chun LUO Zhao-feng WANG Ju-ying MA De-yi |
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| 作者单位： | [1]National Marine Environmental Monitoring Center, Dalian 116023, P. R. China [2]School of Life Science, University of Science and Technology of China, Hefei 230027, P. K. G China . [3]School of Environmental Science and Engineering, Dalian Maritime University, Dalian 116023, P. R.China |
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| 基金项目： | Supported by the State Key Laboratory of the State 0ceanographic Administration of China（ No. 200401 ）. |
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| 摘 要： | In this study, surface plasmon resonance （SPR） for monitoring 17β-eatradiol （E2） was developed. The small molecule E2 was immobilized on a CM5 sensor chip for an indirect competitive immunoassay to detect E2. The SPR response bahed on the antigen-antibody reaction was measured by injecting the sample solution into the flow system. The limitation of detection was 0.445 μg/L. The developed SPE-SPR system was applied to analyze the seawater samples. Recovery of E2 was 91.6%-93. 1%. Relative standard deviations（RSD） for the E2 assay were between 10.9%-15.1% （n = 3）. The range of determination of E2 samples from the sewage in the coastal marine environ-ment was between ND（lower than detection limit） and ca. 11.78 ng/L.
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| 关 键 词： | 表面等离子体共振技术 免疫测定 海洋环境 污水监测 |
| 收稿时间： | 14 August 2006 |
Analysis of 17β-Estadiol from Sewage in Coastal Marine Environment by Surface Plasmon Resonance Technique |
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| ZHANG Wei-wei CHEN Yu-chun LUO Zhao-feng WANG Ju-ying MA De-yi.Analysis of 17β-Estadiol from Sewage in Coastal Marine Environment by Surface Plasmon Resonance Technique[J].Chemical Research in Chinese University,2007,23(4):404-407. |
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| Authors: | ZHANG Wei-wei CHEN Yu-chun LUO Zhao-feng WANG Ju-ying MA De-yi |
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| Institution: | 1. National Marine Environmental Monitoring Center, Dalian 116023, P. R. China;2. School of Life Science, University of Science and Technology of China, Hefei 230027, P. R. China;3. School of Environmental Science and Engineering, Dalian Maritime University, Dalian 116023, P. R. China |
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