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Urease adsorption and activity on magnetite nanoparticles functionalized with monofunctional and bifunctional surface layers |
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| Authors: | Roman P Pogorilyi Inna V Melnyk Yuriy L Zub Guliam A Seisenbaeva Vadim G Kessler Mykola M Shcherbatyik Aljoša Košak Aleksandra Lobnik |
| Institution: | 1. Chuiko Institute of Surface Chemistry, National Academy of Sciences of Ukraine, 17, General Naumov Street, Kyiv, 03164, Ukraine 2. Deparment of Chemistry, Swedish University of Agricultural Sciences, 750 07, Uppsala, Sweden 3. Kholodnyi Institute of Botany, National Academy of Sciences of Ukraine, 2, Tereshchenkivska Street, Kyiv, 01601, Ukraine 4. Faculty of Mechanical Engineering, Centre for Sensor Technology, University of Maribor, Smetanova 17, 2000, Maribor, Slovenia |
| Abstract: | The surface of magnetite nanoparticles was coated with functional polysiloxane layers using reaction of hydrolytic copolycondensation of tetraethoxysilane and 3-aminopropyltriethoxysilane (or N-3-trimethoxysilylpropyl]ethylendiamine), and also that of tetraethoxysilane, 3-aminopropyltriethoxysilane and methyltriethoxysilane (or n-propyltriethoxysilane). It was shown that these functionalized magnetically controllable particles (about 60–150 nm in size as aggregates), as opposed to magnetite, adsorb urease well from aqueous solutions (up to 1 g/g), and that the level of residual activity of adsorbed layers is up to 84 % in the case of a bifunctional sample. It was established that the activity of immobilized urease is normally gradually reduced during storage of the samples, but in the case of ethylenediamine functional group is not decreased for 45 days. The synthesized samples are promising for use as magnetically directed biocatalysts. |
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