Detection and assessment of citrus tristeza virus antigens by computer-aided scanning densitometry |
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Authors: | Rashid A Zeineh George Kyriakidis Roger Acey Christopher Smith |
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Institution: | (1) Biomed Instruments, Inc., 1020 S. Raymond Avenue, #B, 92631 Fullerton, CA;(2) Department of Chemistry, California State University, 90840 Long Beach, CA |
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Abstract: | Citrus tristeza virus (CTV) extracted from Etrog citron (C.medica L.) was immunoprecipitated. The immunoprecipitate was fractionated by SDS-PAGE and western blotted onto nitrocellulose. The CTV
antigens were determined by immunoblot analysis using rabbit anti-CTV IgG, and the protein-band pattern exhibited on the nitrocellulose
was assessed by soft-laser scanning densitometry. The densitometric tracing revealed the presence of bands that were not visible
to the naked eye. Using the superimposition mode of the instrument, it was also revealed that the protein-band patterns of
different CTV samples were not identical. Computer-aided soft-laser scanning densitometry proved to be a powerful approach
in the detection and assessment of protein bands revealed on nitrocellulose immunoblots, which we were previously unable to
do employing conventional methods. |
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Keywords: | Etrog citron immunobloting soft-laser scanning densitometry citrus tristeza virus antigens detection and assessment of by computer-aided scanning densitometry |
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