| 基于固定化酶的流动荧光法测定血清中的葡萄糖 |
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| 引用本文： | 宫志龙,章竹君.基于固定化酶的流动荧光法测定血清中的葡萄糖[J].分析化学,1996,24(9):998-1001. |
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| 作者姓名： | 宫志龙 章竹君 |
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| 作者单位： | 南京大学化学系!南京，210008，陕西师范大学化学系!西安，710062，陕西师范大学化学系!西安，710062 |
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| 摘 要： | 本文将固定化酶柱，光纤以及停流技术结合起来，研究了以廉价而易得的硫胺素为荧光底物的葡萄糖的荧光分析，提出了一种快速、简便、精确地测定葡萄糖的新方法。其线性范围为１．０￣６０．０ｍｇ／Ｌ，线性相关系数为０．９９９，检测限为０．１５ｍｇ／Ｌ。以５．０ｍｇ／Ｌ的葡萄糖作精度试验，ＲＳＤ％＝２．１％（ｎ＝１１）。该方法已成功地用于血清中葡萄糖的测定，结果与光度法相符。
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| 关 键 词： | 葡萄糖氧化酶 荧光分析 葡萄糖 血清 固定化酶 |
Fluorimetric Determination of Glucose in Serum Based on Immobilized Enzymes |
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| Gong Zhilong.Fluorimetric Determination of Glucose in Serum Based on Immobilized Enzymes[J].Chinese Journal of Analytical Chemistry,1996,24(9):998-1001. |
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| Authors: | Gong Zhilong |
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| Abstract: | A new Fluorescence method for the determination of glucose has been proposed.It combines immobilized enzyme column, optical fiber and stopped-flow technique together.Thiamine of is used as a fluorimetric substrate which is cheap and easily available. The linear range of determinaiton is 1.0~60. 0 mg/L with a correlation coefficient of 0. 999 and a detection limit of 0. 15 mg/L. The method has been applied to the determination of glucose in human serum. Results are in agreement with those by spectrophotometry. |
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| Keywords: | Glucose oxidase horseradish peroxidase fluorimetry glucose |
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