Study of zinc-induced changes in lymphocyte membranes using atomic force microscopy,luminescence, and light scattering methods |
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| Authors: | D S Filimonenko A Ya Khairullina V M Yasinskii N M Kozlova G P Zubritskaja E I Slobozhanina |
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| Institution: | (1) Physical Chemistry Department, Faculty of Pharmacy, University of Barcelona, Associated Unit to the CSIC: Peptides and Proteins: Physicochemical Studies, IN2UB Av. Joan XXIII s/n, 08028 Barcelona, Spain;(2) Department of Food Science Quality & Technology, University of Copenhagen, Rolighedsvej 30, 1958 Frederiksberg-C, Denmark;(3) Unit of Synthesis and Biomedical Application of Peptides, Department of Biomedical Chemistry, IQAC-CSIC, Jordi Girona 18, 08034 Barcelona, Spain |
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| Abstract: | Changes in the surface structure of lymphocyte membranes exposed to various concentrations of zinc ions are studied. It is
found by atomic force microscopy that increasing the concentration of zinc ions leads to a reduction in the correlation length
of the autocorrelation function of the roughness profile of a lymphocyte compared to control samples; this may indicate the
existence of fine structure in the membrane surface. Fluorescence markers are used to observe a reduction in the microviscosity
of the lipids in the outer monolayer of the lipid bilayer after lymphocytes are exposed to Zn ions, as well as the exposure
of phosphatidylserine on the surface membrane, and the oxidation of HS-groups of membrane proteins. Calculations of the absorption
coefficients of lymphocytes modified with zinc reveal the existence of absorption bands owing to the formation of metal-protein
complexes and zinc oxide nanoparticles. These results indicate significant changes in the structural and functional state
of lymphocyte membranes exposed to zinc ions. |
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