| X射线和过氧化氢引起的Hela细胞染色质损伤的微区拉曼光谱研究 |
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| 引用本文： | 陈玉玲,沈恂,李心露,田敬东,曾佩,郁鉴源. X射线和过氧化氢引起的Hela细胞染色质损伤的微区拉曼光谱研究[J]. 光散射学报, 1992, 0(1) |
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| 作者姓名： | 陈玉玲 沈恂 李心露 田敬东 曾佩 郁鉴源 |
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| 作者单位： | 中国科学院生物物理所，中国科学院生物物理所，中国科学院生物物理所，中国科学院生物物理所，清华大学化学系，清华大学化学系 北京 100101，北京 100101，北京 100101，北京 100101，北京 100084，北京 100084 |
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| 摘 要： | 我们成功地用微区拉曼光谱探针技术获得了培养单个活体 Hela 细胞核中染色质的拉曼光谱。从光谱中我们看到正常 Hele 细胞核蛋白的二级结构为 a 螺旋,DNA 为 A 型构象。经10~(-3)moI/L 过氧化氢处理20分钟及8Gy x 射线照射后的 Hela 细胞核蛋白的二级结构出现反平行β折叠,DNA 出现 B 型构象。这种结果与我们的假设是相符合的。
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| 关 键 词： | Hela 细胞 拉曼散射 |
The in vivo Micro-Raman spectroscopic study on the damage of chromosomes in HeLa cells induced by X-ray and hydrogen peroxide |
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| Chen Yuling Shen Xun LiXinyuan Tian Jingdong. The in vivo Micro-Raman spectroscopic study on the damage of chromosomes in HeLa cells induced by X-ray and hydrogen peroxide[J]. Chinese Journal of Light Scattering, 1992, 0(1) |
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| Authors: | Chen Yuling Shen Xun LiXinyuan Tian Jingdong |
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| Abstract: | Well resolved Raman spectra of the in vivo chromosomes were obtained for single HeLa cells and used as the sensitive measure of structural changes to study the damage induced by X-ray irradiation and/or H_2O_2.based on the analysis and comparison of the scatter-ing lines of the chromosomal DNA and proteins,it was found that:(1)In untreated cells,the chromosomal proteins and DNA exhibit a α-helical and A-form conformation respectively; (2)Either treatment with 10~(-3) mol/L H_2O_2 or 8Gy 200KV X ray irradiation resulted in the appearance of β-Sheet in conformation of proteins and of B-form in the conformation of DNA. |
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| Keywords: | Raman spectrum Hela cell |
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