Excited-State Dynamics of Proflavine after Intercalation into DNA Duplex |
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| Authors: | Jie Zhou Yanyan Jia Xueli Wang Menghui Jia Haifeng Pan Zhenrong Sun Jinquan Chen |
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| Affiliation: | 1.State Key Laboratory of Precision Spectroscopy, East China Normal University, Shanghai 200241, China;2.Key Laboratory for Advanced Materials, Feringa Nobel Prize Scientist Joint Research Center, Institute of Fine Chemicals, School of Chemistry & Molecular Engineering, East China University of Science and Technology, Shanghai 200237, China;3.Collaborative Innovation Center of Extreme Optics, Shanxi University, Taiyuan 030006, China |
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| Abstract: | ![]()
Proflavine is an acridine derivative which was discovered as one of the earliest antibacterial agents, and it has been proven to have potential application to fields such as chemotherapy, photobiology and solar-energy conversion. In particular, it is well known that proflavine can bind to DNA with different modes, and this may open addition photochemical-reaction channels in DNA. Herein, the excited-state dynamics of proflavine after intercalation into DNA duplex is studied using femtosecond time-resolved spectroscopy, and compared with that in solution. It is demonstrated that both fluorescence and the triplet excited-state generation of proflavine were quenched after intercalation into DNA, due to ultrafast non-radiative channels. A static-quenching mechanism was identified for the proflavine-DNA complex, in line with the spectroscopy data, and the excited-state deactivation mechanism was proposed. |
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| Keywords: | excited-state dynamics proflavine DNA duplex femtosecond transient-absorption fluorescence-quenching |
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