内毒素肺损伤对多形核白细胞化学发光的影响

本文研究了绵羊内毒素肺损伤对全血多形核白细胞(PMN)化学发光的影响;比较了正常混合血浆正常自体血浆和自体致伤血浆调理酵母多糖对发光的影响;观察了致伤后的血浆和肺淋巴液的发光促进活性.结果表明,内毒素致伤后,PMN的化学发光峰时无显著变化,而峰值于4h前呈直线上升,4h达高峰,而后逐渐减弱,但至24h仍明显大于伤前值.三种不同血浆调理酵母多糖对PMN发光无明显影响.致伤后期(2h后)的血浆和肺淋巴液无发光促进活性.这些结果提示,一次性微量的大肠杆菌内毒素对PMN的发光具有强烈和持久的促进作用,而对血浆的调理能力无明显影响.

EFFECTS OF ENDOTOXIN-INDUCED LUNG INJURY ON CHEMILUMINESCENCE OF POLYMOPHONEUCLEAR LEUKOCYTES

Effects of endotoxin-induced lung injury on chemiluminescence(CL) of polymophoneuclear leukocytes (PMNs) are described. In 24 hours after endotoxin injection the peak value of CL of PMNs enhanced markedly though the peak time had no change. Zymosans opsonized by normally pooled plasma and normal self-plasma as well as injuried self-plasma respectively had no effects on CL. The plasma and lung lymph after 2 hours of injury had no activity of promoting CL. These results suggest that a single dose of E. Coli endotoxin should have a strong and lasting effect of activating PMNs and that the Opsonic activity in plasma have no change.