|
|||||
|
|
Functionalised electrode array for the detection of nitric oxide released by endothelial cells using different NO-sensing chemistries |
|
| Authors: | Joshua?Oni Alain?Pailleret Sonnur?Isik Nizam?Diab Ina?Radtke Andrea?Bl?chl Michael?Jackson Fethi?Bedioui |
| Institution: | (1) Anal. Chem. – Elektroanalytik & Sensorik, Ruhr-Universität Bochum, Universitätsstr 150, 44780 Bochum, Germany;(2) Laboratoire d Electrochimie et de Chimie Analytique (UMR CNRS-ENSCP 7575), Ecole Nationale Supérieure de Chimie de Paris, 11 Rue Pierre et Marie Curie, 75231 Paris, Cedex 05, France;(3) Lehrstuhl für Molekulare Neurobiochemie, Ruhr-Universität Bochum, Universitätsstr. 150, 44780 Bochum, Germany;(4) Central Research Laboratories Limited, Dawley Road, Hayes, Middlesex, UK;(5) Present address: Chemistry Department, University of Lagos, Akoka, Lagos, Nigeria |
| Abstract: | In a preliminary study aimed at developing strategies for the simultaneous detection of various biologically important molecules, a procedure is described that allows the electrochemical detection of nitric oxide (NO) released by a population of human umbilical vein endothelial cells (HUVEC) by using an array of electrodes comprising three individually addressable electrodes. Each electrode in the array was modified with a different NO-sensitive electrocatalyst, thereby demonstrating the possibility of modifying the individual electrodes in an array with different sensing chemistries. This study opens a doorway to the development of arrays of electrodes for the simultaneous detection of multiple analytes in a complex environment by suitably tailoring the sensitivity and selectivity of each electrode in the array to a specific analyte in the test medium. |
| Keywords: | Nitric oxide Electrodes array Electrocatalysis Chemically modified electrodes HUVEC |
| 本文献已被 PubMed SpringerLink 等数据库收录！ | |