| 用毛细管气相色谱法测定多糖中单糖的组成 |
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| 引用本文： | 徐桂云,陈汝贤,常理文. 用毛细管气相色谱法测定多糖中单糖的组成[J]. 分析测试学报, 2000, 19(3): 71-73 |
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| 作者姓名： | 徐桂云 陈汝贤 常理文 |
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| 作者单位： | 中国科学院,分子科学中心,化学研究所,北京,100080;中国医学科学院,生物技术研究所,北京,100050 |
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| 摘 要： | 报道了测定多糖中单糖组成的糖醇乙酸酯的毛细管气相色谱分析方法。使用ＯＶ－２２５毛细管气相色谱柱分离了１１种单糖的糖醇乙酸酯衍生物,在０．２～１．６８ｇ／Ｌ质量浓度范围内,１１种单糖定量校正曲线的线性关系廊。应用该法测定了胡麻我发多糖和少 我中单糖的组成。为这些药物多糖的基础研究提供了有用的信息。
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| 关 键 词： | 多糖 乙酰 气相色谱分析 单糖 |
| 修稿时间： | 1999－07－25 |
Component Analysis of Monosaccharides in Polysaccharides by Capillary Gas Chromatography |
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| XU Gui-yun,CHEN Ru-xian,CHANG Li-wen. Component Analysis of Monosaccharides in Polysaccharides by Capillary Gas Chromatography[J]. Journal of Instrumental Analysis, 2000, 19(3): 71-73 |
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| Authors: | XU Gui-yun CHEN Ru-xian CHANG Li-wen |
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| Affiliation: | XU Gui-yun, CHANG Li-wen;( Center for Molecular Science, Institute of Chemistry, Chinese Academy of Sciences,Beijing 100080, China);CHEN Ru-xian;(Institute of Medical Biotechnology, Chinese Academy of Medical Sciences, Beijing 100050,China) |
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| Abstract: | Determination of monosaccharides in polysaccharides is reported by gas chromatography with OV-225 capillary column. Monosaccharides from the polysaccharides hydrolyzed in aqueous trifluoroacetic acid are reduced by NaBH4. Reduced monosaccharides are derivatized into sugar alcohol acetates with acetic anhydride pyridine before determination. The method was used to determine monosaccharides in polysaccharides from flak, angelica sinensis, and laminaria. The results showed that the monosaccharides in polysaccharide included rhamnose, arabinose, xylose, galactose and glucose for flax; rhamnose, arabinose, galactose and glucose for angelica sinensis; fucose, galactose, mannose, glucose and xylose for laminaria. |
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| Keywords: | Polysaccharide Acetylation Gas chromatography Monosaccharide |
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