World-to-chip microfluidic interface with built-in valves for multichamber chip-based PCR assays |
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Authors: | Oh Kwang W Park Chinsung Namkoong Kak Kim Jintae Ock Kyeong-Sik Kim Suhyeon Kim Young-A Cho Yoon-Kyoung Ko Christopher |
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Institution: | Bio Lab, Samsung Advanced Institute of Technology, P.O. Box 111, Suwon 440-600, Korea. MrOhio@Samsung.com |
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Abstract: | We report a practical world-to-chip microfluidic interfacing method with built-in valves suitable for microscale multichamber chip-based assays. One of the primary challenges associated with the successful commercialization of fully integrated microfluidic systems has been the lack of reliable world-to-chip microfluidic interconnections. After sample loading and sealing, leakage tests were conducted at 100 degrees C for 30 min and no detectable leakage flows were found during the test for 100 microchambers. To demonstrate the utility of our world-to-chip microfluidic interface, we designed a microscale PCR chip with four chambers and performed PCR assays. The PCR results yielded a 100% success rate with no contamination or leakage failures. In conclusion, we have introduced a simple and inexpensive microfluidic interfacing system for both sample loading and sealing with no dead volume, no leakage flow and biochemical compatibility. |
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