Polypyrrole modified stainless steel frits for on-line micro solid phase extraction of ochratoxin A |
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Authors: | Jorn?C?C?Yu Email author" target="_blank">Edward?P?C?LaiEmail author |
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Institution: | (1) Ottawa-Carleton Chemistry Institute, Department of Chemistry, Carleton University, Ottawa, ON, K1S 5B6, Canada |
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Abstract: | Polypyrrole (PPy) was electrochemically synthesized on stainless steel frits as a sorbent for the micro solid phase extraction (SPE) of ochratoxin A (OTA). Using 20 l of standard solution under a fast flow rate of 0.5 ml/min, 80% recovery of OTA was achieved in the concentration range from 0.1–10 pg/l. This good recovery was achieved within a short residence time of 1.2 s. A binding capacity of 1 ng OTA was estimated for each PPy-modified frit, or 2 ng OTA for two frits in series. The bound OTA could be pulsed eluted (PE) with 20 l of 1% triethylamine in acetonitrile. On-line coupling of this PPy-on-a-frit and PE technique to high performance liquid chromatography (HPLC) was straightforward. On-line SPE-PE-HPLC results clearly demonstrated the capability of PPy-on-a-frit to bind OTA in the presence of red wine, beer, and orange juice components. |
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Keywords: | Polypyrrole Stainless steel frit Micro solid phase extraction Ochratoxin A Pulsed elution High performance liquid chromatography |
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