Porous structure of the monolithic sorbent and separation properties of monolithic capillary columns in gas and liquid chromatography |
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Authors: | Korolev A A Shiryaeva V E Popova T P Viktorova E N Kurganov A A |
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Institution: | 1.A. V. Topchiev Institute of Petrochemical Synthesis, Russian Academy of Sciences, 29 Leninsky prosp., 119991, Moscow, Russian Federation ; |
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Abstract: | Macroporous polymer based on polydivinylbenzene was used for the preparation of monolithic capillary columns with the diameter
from 0.01 to 0.53 mm for separations by gas and liquid chromatography. The separation properties of the columns were studied
by analysis of model systems of aromatic (in liquid chromatography) and light (in gas chromatography) hydrocarbons. The permeability
was determined and the C parameter of the Van-Deemter equation was found for each column. The permeability of the majority of columns determined by
gas chromatography is independent of the column diameter. The permeability of the same columns in liquid chromatography is
also almost constant for the columns 0.53–0.1 mm in diameter; however, the permeability decreases sharply on going to columns
of smaller diameter. In gas chromatography the value of the C parameter reflecting the effect of the mass transfer of the sorbate between the mobile and stationary phases on the smearing
of a chromatographic peak in the column approximately the same for all columns. In liquid chromatography the value of the
C coefficient in the Van-Deemter equation for the same capillary columns changes with a change in the column diameter and reaches
a minimum for the columns 0.1 mm in diameter. The differences observed for the characteristics of the columns in gas and liquid
chromatography are due to different structures of the macroporous monolith formed in columns of different diameter and to
the effect of solvation of the monolith by the mobile phase under the conditions of liquid chromatography. |
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