| 带有位阻基团的钌多吡啶配合物与DNA键合及其光断裂DNA性质的研究 |
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| 引用本文： | 蒲小华,陈绘丽,韩高义,杨频.带有位阻基团的钌多吡啶配合物与DNA键合及其光断裂DNA性质的研究[J].化学学报,2007,65(15):1464-1468. |
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| 作者姓名： | 蒲小华 陈绘丽 韩高义 杨频 |
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| 作者单位： | 1. 山西大学分子科学研究所,化学生物学与分子工程教育部重点实验室,太原,030006;宝鸡文理学院化学化工系,宝鸡,721007
2. 山西大学分子科学研究所,化学生物学与分子工程教育部重点实验室,太原,030006 |
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| 摘 要： | 合成了两个钌多吡啶配合物Ru(bpy)2DMNP](C1O4)2 (Ru1)和Ru(bpy)2BOPIP](C1O4)2 (Ru2), 应用元素分析、核磁共振对配合物结构进行了表征, 通过电子吸收光谱、荧光光谱、粘度实验以及凝胶电泳技术对配合物与DNA相互作用的性质进行了研究. 结果表明, 配合物与DNA分子之间以插入模式结合. 在紫外光照下, 两种配合物均能使质粒pBR322DNA断裂, 机理研究表明, 其光断裂DNA的活性氧化物种为单线态氧.
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| 关 键 词： | 钌多吡啶配合物 DNA 光断裂 插入作用 |
| 收稿时间： | 2006-5-9 |
| 修稿时间： | 2006-05-092007-04-23 |
DNA-binding and Photocleavage Studies on Polypyridyl Ruthe-nium(II) Complexes Containing Steric Hinderance Group |
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| PU Xiao-Hua,CHEN Hui-Li,HAN Gao-Yi,YANG Pin.DNA-binding and Photocleavage Studies on Polypyridyl Ruthe-nium(II) Complexes Containing Steric Hinderance Group[J].Acta Chimica Sinica,2007,65(15):1464-1468. |
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| Authors: | PU Xiao-Hua CHEN Hui-Li HAN Gao-Yi YANG Pin |
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| Institution: | a Key Laboratory of Chemical Biology and Molecular Engineering of Education Ministry, Institute of Molecular Science, Shanxi University, Taiyuan 030006;b Department of Chemistry, Baoji University of Arts and Science, Baoji 721007 |
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| Abstract: | The polypyridyl ruthenium(II) complexes of Ru(bpy)2DMNP]2＋ and Ru(bpy)2BOPIP]2＋ have been synthesized and their structures have been ascertained with elemental analysis and nuclear magnetic resonance spectra. The characteristics of the interaction of the complexes with CT-DNA were examined by Ultra-Vis absorption spectra, fluorescent spectra, viscosity measurements and gel electrophoresis technique. All data indicate that both complexes bind to double-stranded CT-DNA via the intercalative mode and Ru(bpy)2DMNP]2＋ binds more avidly to CT-DNA than Ru(bpy)2BOPIP]2＋. Noticeably, upon irradiation at 365 nm, the two complexes have been found to efficiently photocleave plasmid DNA, and the active oxygen species for the DNA cleavage in these complexes is singlet molecular oxygen. |
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| Keywords: | polypyridyl ruthenium(II) complex DNA photocleavage intercalation |
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