| 基于纳米ZnO/壳聚糖复合膜DNA电化学传感器用于HIV基因的免标记检测 |
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| 引用本文： | 刘志敏,李哲建,康宁.基于纳米ZnO/壳聚糖复合膜DNA电化学传感器用于HIV基因的免标记检测[J].分析科学学报,2010,26(1). |
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| 作者姓名： | 刘志敏 李哲建 康宁 |
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| 作者单位： | 河南工业大学化学化工学院,河南郑州,450052 |
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| 基金项目： | 河南省教育厅高校青年骨干教师基金(No.001135);;河南省教育厅自然科学基金(No.2008B150006) |
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| 摘 要： | 以室温固相合成法制备纳米ZnO,通过壳聚糖(CHIT)的成膜效应将纳米ZnO固定在玻碳电极(GCE)表面,制得的ZnO/CHIT/GCE电极成为DNA固定和杂交的良好平台。DNA的固定和杂交通过电化学交流阻抗进行表征。以电化学交流阻抗免标记法检测目标DNA,固定于电极表面的DNA探针与目标DNA杂交后使电极表面的电子传递电阻增大,以此作为检测信号可以高灵敏度地测定目标DNA。电化学阻抗谱检测人类免疫缺陷病毒(HIV)基因片段的线性范围为2.0×10-11~2.0×10-6mol/L,检出限为2.0×10-12mol/L。
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| 关 键 词： | 纳米ZnO DNA 电化学交流阻抗 人类免疫缺陷病毒基因 |
Lable-free Detection of HIV Based on Nano-ZnO/Chitosan Composite Film Modified DNA Electrochemical Biosensor |
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| LIU Zhi-min,LI Zhe-jian,KANG Ning.Lable-free Detection of HIV Based on Nano-ZnO/Chitosan Composite Film Modified DNA Electrochemical Biosensor[J].Journal of Analytical Science,2010,26(1). |
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| Authors: | LIU Zhi-min LI Zhe-jian KANG Ning |
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| Institution: | College of Chemistry and Chemical Engineering;Henan University of Technology;Zhengzhou 450052 |
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| Abstract: | Nano-ZnO was synthesized by solid state reaction and was immobilized on glass carbon electrode via the film forming by chitosan(CHIT) for DNA immobilization and hybridization.The electrochemical impedance spectroscopy(EIS) was used to characterize the immobilization and hybridization.EIS was further applied to label-free detection of the target DNA based on the increase of the electron transfer resistance(Ret) of the electrode surface after the hybridization of the probe DNA with the target DNA.Under the op... |
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| Keywords: | Nano-ZnO DNA Electrochemical impedance spectroscopy HIV gene |
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