High-performance liquid chromatographic profiles of low-molecular-weight,u.v.-absorbing compounds in the sera of beagles exposed to cigarette smoke

High-performance liquid chromatography with a nonpolar stationary phase was used to determine the low-molecular-weight, u.v.-absorbing compounds in the sera of male beagles exposed to cigarette smoke. The sera from three groups of tracheostomized beagles were processed. The ChromatographiC profiles of the beagles exposed to the smoke of low-nicotine cigarettes for 610–750 days were not significantly different from the profiles of beagles identically exposed to the smoke of high-nicotine cigarettes. However, several important differences were determined between the profiles of sham controls and the dogs exposed to cigarette smoke. Most notably, a two-fold elevation of serum inosine was found in all beagles exposed to cigarette smoke.