Electrochemical behavior of hemoglobin in neutral surfactants with different poly(ethylene oxide) unit lengths adsorbed on an electrode

The direct electron transfer and adsorption behavior of hemoglobin(Hb) in a series of surfactants with different poly(ethylene oxide)(PEO) unit lengths on a glassy carbon electrode have been studied.With a surfactant of appropriate PEO unit length,the surfactant film-modified electrode exhibited a more stable adsorption state with a larger surface coverage of Hb and a more positive formal potential,which can be attributed to the effect of hydrogen bonding between proteins and surfactants.The electrochemical behavior of surfactants with different PEO unit lengths is discussed in detail.Moreover,UV-visible spectroscopy demonstrated that the structure of Hb was not destroyed in the surfactant films.The electrocatalytic activity of hydrogen peroxide on three neutral surfactant-modified electrodes has also been investigated.