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Application of a rapid screening method to detect irradiated meat in Brazil |
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| Authors: | Anna Lucia C. H. Villavicencio, Jorge Mancini-Filho,Henry Delinc e |
| Affiliation: | a IPEN/CNEN-SP, Department of Engineering and Industrial Applications, Travessa R, No 400 — Cidade Universitária, 05508-910, São Paulo CEP, Brazil b F.C.F. USP, Food and Experimental Nutrition Department, São Paulo, Brazil c Federal Research Centre for Nutrition, BFE, Haid-und-Neu-Str. 9, D-76131, Karlsruhe, Germany |
| Abstract: | Based on the enormous potential for food irradiation in Brazil, and to ensure free consumer choice, there is a need to find a convenient and rapid method for detection of irradiated food. Since treatment with ionising radiation causes DNA fragmentation, the analysis of DNA damage might be promising. In this paper, the DNA Comet Assay was used to identify exotic meat (boar, jacaré and capybara), irradiated with 60Co gamma rays. The applied radiation doses were 0, 1.5, 3.0 and 4.5 kGy. Analysis of the DNA migration enabled a rapid identification of the radiation treatment. |
| Keywords: | Food irradiation detection Meat DNA Comet assay |
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