| 花生致敏糖蛋白Ara h1糖链决定簇的质谱分析 |
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| 引用本文： | 彭艺芳,王承健,王晶晶,李玲梅,晋万军,强珊,师红丹,张英,黄琳娟,王仲孚.花生致敏糖蛋白Ara h1糖链决定簇的质谱分析[J].高等学校化学学报,2016(9):1622-1628. |
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| 作者姓名： | 彭艺芳 王承健 王晶晶 李玲梅 晋万军 强珊 师红丹 张英 黄琳娟 王仲孚 |
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| 作者单位： | 西部资源生物与现代生物技术教育部重点实验室，陕西省生物技术重点实验室，西北大学生命科学学院，西安710069 |
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| 基金项目： | 国家自然科学基金(批准号:21375103;31370804)资助. Supported by the National Natural Science Foundation of China(.21375103 |
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| 摘 要： | 以花生种子总蛋白及其主要致敏糖蛋白Ara h1为研究对象,采用"一釜法"对蛋白上的糖链进行释放并同时进行衍生化标记,通过C18固相萃取柱纯化,以电喷雾质谱(ESI-MS)、多级串联质谱(MSn)和亲水性液相色谱-质谱联用(HILIC-MS)进行结构解析和定量分析.结果表明,蛋白Ara h1共有10条N-糖链,其中7条为高甘露糖型,2条为木糖修饰,另外1条为与过敏原相关的核心α1,3-Fuc修饰N-糖链,其含量约占总糖链的12.45%.
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| 关 键 词： | 花生过敏 致敏糖蛋白Ara h1 糖链结构 电喷雾质谱 亲水性液相色谱-质谱联用 |
Analysis of Antigenic Determinant Glycans of Peanut Allergy Glycoprotein Ara h1 by Mass Spectrometry |
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| Abstract: | Peanut seed total protein and glycoprotein Ara h1, which can cause allergic reactions, were chosen as the target materials in this study. Firstly, the glycans were released and labeled with 1-phenyl-3-methyl-5-pyrazolone( PMP) by recently developed “one-pot” method. Subsequently, the glycans were fractionated and enriched through C18 solid phase extraction column. Finally, the obtained glycans were analysed by electros-pray ionization mass spectrometry ( ESI-MS), MSn and Hydrophilic interaction liquid chsomatography-mass spectrometry( HILIC-MS) . The results indicated that Ara h1 has ten N-glycans, including seven high-mannose type, two ones with xylose modification, and one allergenic core α1,3-fucosylated N-glycan, the content of which is 12. 45% of total glycans. |
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| Keywords: | Peanut allergy Allergenic glycoprotein Ara h1 Glycan structure Electrospray ionization mass spectrometry( ESI-MS) Hydrophilic interaction liquid chsomatography-mass spectrometry( HILIC-MS) |
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