Antioxidative Effect of Schiff Bases with o-Hydroxybenzylidene Groupon Free Radical Induced Hemolysis of Human Red Blood Cell

The hemolysis of human red blood cells was initiated by a water-soluble free radical initiator, 2,2′-azobis-(2-amidinopropane hydrochloride)(AAPH), at 37 ℃ in phosphate buffered saline(pH=7.4). The respective addition of 1-N-(o-hydroxybenzylidene)amino]tetradecane(TDCA), 1,2-diN-(o-hydroxybenzylidene)amino]cyclohexane(DACH), 4-N-(o-hydroxybenzylidene)amino]benzoic acid(PABA), 4-nitroN-(o-hydroxybenzylidene)]aniline(APNA) or N-(o-hydroxybenzylidene)aniline(APA) can all prolong the inhibition period of hemolysis, indicating that the above Schiff bases play an antioxidative role in free radical induced hemolysis. It can be concluded that Schiff base with an alkyl group or a conjugated system in the molecule protect red blood cells against free radical induced hemolysis efficiently. This information may be useful for antioxidant drug design.