新型键合纤维素手性固定相的制备及其拆分性能评价

键合型多糖手性固定相因具有化学稳定性高和溶剂耐受性好的特点而受到研究者的极大关注。采用施陶丁格（Staudinger）反应将6-叠氮-6-脱氧纤维素-3，5-二氯苯基氨基甲酸酯键合到氨丙基硅胶上得到一种新的键合型手性固定相（ImCel），研究了其手性分离性能，并探讨了非常规流动相（如氯仿、四氢呋喃等）的影响。结果表明，在20对手性化合物中，17对在合适的流动相下得到基线分离。ImCel在正相条件下的分离性能优于反相条件，且在含氯仿的流动相中仍对手性化合物表现出良好的分离能力。在分离一系列芴甲氧羰基（fmoc）-氨基酸衍生物时，ImCel与键合6-叠氮-6-脱氧纤维素-3，5-二甲基苯基氨基甲酸酯的手性固定相表现出互补性，出现了固定相改变引起的对映体洗脱反转现象。本研究丰富了键合型多糖手性固定相的种类和合成方法，为开发新的键合型手性固定相提供了参考。

Preparation of a new immobilized cellulose-based chiral stationary phase and its enantioseparation behaviors

The immobilized polysaccharide-based chiral stationary phase has attracted considerable attention over the past decades due to its high chemical stability, good solvent resistance, great enantioseparation ability, etc. In this study, a new immobilized cellulose chiral stationary phase (denoted as ImCel) was prepared through the Staudinger reaction of 6-azido-6-deoxy-cellulose-3,5-dichlorophenylcarbamate and aminopropyl silica gel. The enantioseparation performance of the ImCel for 20 pairs of chiral analytes and the effect of non-standard solvents have been investigated by high performance liquid chromatography. Baseline separations of 17 pairs of enantiomers were achieved on the ImCel. The separation ability of the ImCel in the normal mode was much better than in reversed mode. In addition, the ImCel showed good chemical stability in the non-standard mobile phase due to the covalent bonds between the cellulose chiral selectors and silica support. Moreover, it exhibited complementarity with another immobilized-cellulose chiral stationary phase containing 3,5-dimethylphenylcarbamate groups for the separation of a series of 9-fluorenylmethyloxycarbonyl (fmoc)-derived amino acids. The reversal of enantiomer elution order induced by the difference of the substituents in chiral stationary phase was observed under the same chromatographic conditions. In brief, a new immobilized cellulose chiral stationary phase with high stability and good separation performance was developed in this work.