| Determination of Vitamin C in Plasma and Dialysate from Uremia Patientsby High Performance Liquid Chromatography with Electrochemical Detection |
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| 引用本文： | 朱秋毓,丁峰,俞茂华,顾勇,林善锬. Determination of Vitamin C in Plasma and Dialysate from Uremia Patientsby High Performance Liquid Chromatography with Electrochemical Detection[J]. 色谱, 2002, 20(2): 151-155 |
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| 作者姓名： | 朱秋毓 丁峰 俞茂华 顾勇 林善锬 |
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| 作者单位： | 复旦大学医学院附属华山医院肾病科,上海,200040 |
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| 摘 要： | Abstract：Aconvenientandvalidmethodforthedeterminationofascorbicacid(AA)anddehydroascorbicacid(DHAA)inplasmaanddialysatefrompatientswithuremiabyhighperformanceliquidchromatographywithelectrochemicaldetectionisdescribedAmixtureof08g/Lmetaphosphoricacidand
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| 关 键 词： | 高效液相色谱法 电化学检测 抗坏血酸 脱氢抗坏血酸 维生素C 血浆 透析液 尿毒症 测定 临床检验 |
ZHU Qiu-yu, male, born in 1952, technician in charge, Tel:(021)62511127-324. Determination of Vitamin C in Plasma and Dialysate from Uremia Patients by High Performance Liquid Chromatography with Electrochemical Detection |
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| ZHU Qiu-yu,DING Feng,YU Mao-hua,GU Yong,LIN Shan-yan. ZHU Qiu-yu, male, born in 1952, technician in charge, Tel:(021)62511127-324. Determination of Vitamin C in Plasma and Dialysate from Uremia Patients by High Performance Liquid Chromatography with Electrochemical Detection[J]. Chinese journal of chromatography, 2002, 20(2): 151-155 |
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| Authors: | ZHU Qiu-yu DING Feng YU Mao-hua GU Yong LIN Shan-yan |
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| Affiliation: | Department of Nephrology, Hua Shan Hospital, School of Medicine, Fudan University, Shanghai 200040, China. |
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| Abstract: | A convenient and valid method for the determination of ascorbic acid(AA) and dehydroascorbic acid(DHAA) in plasma and dialysate from patients with uremia by high performance liquid chromatography with electrochemical detection is described. A mixture of 0.8 g/L metaphosphoric acid and 18% (volume fraction) perchloric acid was used as a protein precipitant and the extractant for AA from biosamples. It was also a good stabilizer for AA in samples. The proposed method is satisfied for routine screening of vitamin C in clinical applications with a correlation coefficient of more than 0.99 in the range of 2 mumol/L-40 mumol/L AA. The within-day precision was less than 8.9% and 10.55% for AA and DHAA, respectively. The recoveries of AA in plasma and dialysate were over 95% and 78%, respectively. |
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| Keywords: | high performance liquid chromatography electrochemical detection ascorbic acid dehydroascorbic acid vitamin C plasma dialysate uremia |
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