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Trace determination of nitrated polycyclic aromatic hydrocarbons using liquid chromatography with on-line electrochemical reduction and fluorescence detection |
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| Authors: | Ching-Tang Kuo Hong-Wen Chen Shaw-Tao Lin |
| Institution: | a Department of Risk Management, China Medical College, 91 Hsueh-Shih Road, Taichung 404, Taiwan b Institute of Environmental Health, China Medical College, 91 Hsueh-Shih Road, Taichung 404, Taiwan c Department of Environmental Engineering and Health, Yuanpei University of Science and Technology, 306 Yuanpei Street, Hsinchu, Taiwan d Institute of Environmental Engineering, National Chiao Tung University, 75 Po-Ai Street, Hsinchu 300, Taiwan e Department of Applied Chemistry, Providence University, Sha-Lu, Taichung Hsien 433, Taiwan |
| Abstract: | An efficient clean-up procedure coupled with a high performance liquid chromatography (HPLC) with on-line electrochemical (EC) reduction and fluorescence detection (FLD) was developed to quantify nitrated polycyclic aromatic hydrocarbons (NPAHs) in the airborne particulate. In this process, NPAHs were extracted ultrasonically followed by analysis by using a reversed phase column with an aqueous eluent containing 70% aqueous acetonitrile and sodium monocholoroacetate as a buffer solution. The extraction efficiencies were above 83% for 1-nitropyrene and 1,3-dinitropyrene (1,3-DNP) 1,6-DNP, and 1,8-DNP, and calibration graphs were linear with very good correlation coefficients (r>0.999) and the detection limits were in the range of 1.0-2.2 pg for dinitropyrenes and nitropyrene. The proposed method provides a relatively simple and convenient procedure for determining the NPAHs samples in airborne particulate. |
| Keywords: | Nitrated polycyclic aromatic hydrocarbons Ultrasonic extraction High performance liquid chromatography Electrochemical reduction Fluorescence detection |
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