Separation of nonpolar analytes using methanol-water mixtures at elevated temperatures |
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Authors: | Aaryn Jones |
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Institution: | Department of Chemistry, East Carolina University, College of Arts and Sciences, Greenville, NC 27858-4353, USA |
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Abstract: | Pure subcritical water has been found to be an efficient mobile phase for reversed-phase separations of both polar and moderately polar compounds. However, subcritical water must be modified with organic solvent in order to elute nonpolar analytes in an efficient manner. In this study, the separation of polycyclic aromatic hydrocarbons (PAHs), polychlorinated biphenyls (PCBs), and benzene, toluene, and p-xylene (BTX) was performed by using heated methanol-water mixtures as the mobile phase. Temperatures employed in this study ranged from 21 to 140 °C, while the percentage of methanol in the mobile phase ranged from 52 to 90%. The retention times of analytes were matched under different mobile phase conditions by increasing the temperature and decreasing the percentage of methanol in the mobile phase. |
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Keywords: | Analytes Reversed-phase liquid chromatography (RPLC) High-temperature liquid chromatography (HTLC) Polychlorinated biphenyls (PCBs) Polycyclic aromatic hydrocarbon |
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