Direct determination of single-to-double stranded DNA ratio in solution using steady-state fluorescence measurements期刊界 All Journals 搜尽天下杂志传播学术成果专业期刊搜索期刊信息化学术搜索

Direct determination of single-to-double stranded DNA ratio in solution using steady-state fluorescence measurements

Authors:	Beach Lori Schweitzer Claude Scaiano J C

Affiliation:	Department of Chemistry, University of Ottawa, 10 Marie Curie, Ottawa, Ontario, Canada K1N 6N5.

Abstract:	We report a simple and rapid method for quantitation of single-to-double stranded (ss : ds) DNA ratios in solution, using steady-state measurements of fluorescence from two simultaneously excited intercalated dyes; the ratio of fluorescence intensities from PicoGreen (525 nm) and ethidium bromide (610 nm) is directly proportional to the ss : ds DNA ratio.

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