| 流动注射-抑制化学发光法测定银杏叶中总黄酮含量 |
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| 引用本文： | 徐文峰. 流动注射-抑制化学发光法测定银杏叶中总黄酮含量[J]. 分析试验室, 2009, 28(12) |
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| 作者姓名： | 徐文峰 |
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| 作者单位： | 重庆文理学院,永川,402160 |
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| 基金项目： | 重庆市教育委员会科技研究 |
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| 摘 要： | 提出了流动注射-抑制化学发光测定银杏叶中的总黄酮含量的分析方法.它是基于银杏叶中物质黄酮类具有还原性,在碱性条件下还原H_2O_2,抑制鲁米诺-H_2O_2-KIO_4体系的化学发光,其抑制程度的大小与总黄酮的含量成线性关系.方法的线性范围为1.5～30μg/mL,检出限为0.03μg/mL,相对标准偏差(RSD)为1.2%,采样频率为240次/h,回收率为101%～104%.
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| 关 键 词： | 流动注射-抑制化学发光 银杏叶 总黄酮 |
Determination of total flavonoids in ginkgo leaves using flow-injection inhibitory chemiluminescence |
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| XU Wen-feng. Determination of total flavonoids in ginkgo leaves using flow-injection inhibitory chemiluminescence[J]. Chinese Journal of Analysis Laboratory, 2009, 28(12) |
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| Authors: | XU Wen-feng |
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| Affiliation: | XU Wen-feng(School of Chemistry and Environment Engineering,Chongqing University of Arts and Sciences,Chongqing 402160) |
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| Abstract: | A novel flow injection inhibitory chemiluminescence system for the determination of total flavonoids was pre-sented. Flavonoids could inhibit the CL reaction of Luminol-H_2O_2-KIO_4 system in alkaline aqueous solution and the CL intensity was inversely correlated with the flavonoids concentration. The linear range was 1.5～30 μg/mL with a detec-tion limit of 0.03μg/mL, the recoveries were 101%～104%, the sampling frequency was 240 times per hour. The method was applied to the determination of total flavonoids, good results were obtained. |
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| Keywords: | Flow injection inhibitory chemiluminescence Ginkgo leaves Total flavonoids |
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