| HCVNS2基因表达载体的构建及在E.coli中表达 |
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| 引用本文： | 赵月娥,叶林柏,郜金荣,徐进平,刘源洁,吴正辉.HCVNS2基因表达载体的构建及在E.coli中表达[J].武汉大学学报(理学版),2001,47(4):481-484. |
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| 作者姓名： | 赵月娥 叶林柏 郜金荣 徐进平 刘源洁 吴正辉 |
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| 作者单位： | 武汉大学病毒研究所, |
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| 摘 要： | PCR人含有丙肝病毒全长非结构蛋白的载体pBlueBac25中扩增出全长的NS2基因DNA片段，分别克隆到表达载体pQE30和转座载体pFasBacHTb的多隆位点（MCS），PFastNS2通过转座插入穿梭载体Bacmid的表达盒；pQENS2转化JM109菌株，诱导表达出N端含有6个His的全长His的全长NS2蛋白，用Ni-NTA-agarose柱层纯化，获得提纯的全长NS2蛋白。
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| 关 键 词： | HCVSN2 构建载体 纯化 丙型肝炎病毒 NS2基因 NS2蛋白 基因表达 |
| 文章编号： | 0253-9888(2001)04-0481-04 |
| 修稿时间： | 2000年6月25日 |
The Construction of Expression Vector of HCV NS2 and Expression in E. coli |
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| ZHAO Yue-e,YE Lin-bai,GAO Jin-rong,XU Jin-ping,LIU Yuan-jie,WU Zheng-hui.The Construction of Expression Vector of HCV NS2 and Expression in E. coli[J].JOurnal of Wuhan University:Natural Science Edition,2001,47(4):481-484. |
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| Authors: | ZHAO Yue-e YE Lin-bai GAO Jin-rong XU Jin-ping LIU Yuan-jie WU Zheng-hui |
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| Abstract: | Full-length NS2 gene of hepatitis C virus was obtained by PCR,using plasmid pBlueBac25 harboring HCV nonstructrual gene as template.This fragment was cloned into plasmid pQE30 and transposition vector pFastBacHTb obtained pQENS2 and pFastNS2 respectively, pFastNS2 transfected DH10Bac strain and was insert into the Bacmid of Baculovirus genome by tranposition.PQENS2 was transformed JM109 strain. The NS2 protein fused to 6 His peptides at N terminus were expressed in JM109 induced by IPTG and purified by Ni-NTA-agarose. |
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| Keywords: | HCV NS2 construction of vector expression purification |
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