Transient silylation of the guanosine O6 and amino groups facilitates N-acylation |
| |
Authors: | Fan Yupeng Gaffney Barbara L Jones Roger A |
| |
Institution: | Department of Chemistry and Chemical Biology, 610 Taylor Road, Rutgers, The State University of New Jersey, Piscataway, New Jersey 08854, USA. |
| |
Abstract: | reaction: see text] The formation of a guanosine derivative silylated at both the O6 and amino groups was identified by (15)N NMR. This intermediate allows facile reaction with acetyl chloride or phenoxyacetyl chloride to give in high yield the corresponding N-protected guanosine derivatives, suitable for use in RNA synthesis. The acetyl and phenoxyacetyl amino protecting groups are, respectively, 4 and 230 times more labile than the isobutyryl group to methylamine/ethanol deprotection. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|