1-ethynylpyrene-modified guanine and cytosine as optical labels for DNA hybridization |
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| Authors: | Wagner Clemens Rist Manuela Mayer-Enthart Elke Wagenknecht Hans-Achim |
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| Affiliation: | Technical University of Munich, Chemistry Department, Lichtenbergstr. 4, D-85747 Garching, Germany. |
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| Abstract: | 1-ethynylpyrene shows remarkable absorption changes upon DNA hybridization when it is covalently attached to the 8-position of guanine. An absorption band at approximately 420 nm is only present in the duplex, exhibits thermal melting behaviour and provides the basis for a molecular beacon together with 1-ethynylpyrene-modified cytosine. |
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