| 以聚硫堇为电化学探针的非标记型核酸适配体传感器 |
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| 引用本文： | 杨绍明,查文玲,李红,孙清,刘斌,郑龙珍.以聚硫堇为电化学探针的非标记型核酸适配体传感器[J].高等学校化学学报,2013,34(3):551-555. |
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| 作者姓名： | 杨绍明 查文玲 李红 孙清 刘斌 郑龙珍 |
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| 作者单位： | 华东交通大学基础学院化学化工系, 南昌 330013 |
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| 基金项目： | 国家自然科学基金(批准号:21065004);江西省自然科学基金(批准号:2009GQH0022);江西省教育厅科技项目(批准号:GJJ12304)资助 |
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| 摘 要： | 采用电聚合法制备了聚硫堇氧化还原电化学探针, 以金纳米粒子为固定核酸适配体的载体构建了非标记型核酸适配体传感器. 用电化学阻抗谱对传感器的组装过程进行了监测, 用循环伏安法和差分脉冲伏安法考察了传感器的电化学行为. 结果表明, 该传感器对凝血酶的检测在1.0 pg/mL～500 ng/mL范围内呈良好的线性关系, 相关系数为0.998, 检出限为0.38 pg/mL. 该传感器制备简单、 灵敏度高且抗干扰能力强.
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| 关 键 词： | 聚硫堇 金纳米粒子 核酸适配体 凝血酶 传感器 |
| 收稿时间： | 2012-07-20 |
Label-free Aptamer Biosensor with Poly(thionine) as an Electrochemical Probe |
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| YANG Shao-Ming
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ZHA Wen-Ling
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LI Hong
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SUN Qing
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LIU Bin
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ZHENG Long-Zhen.Label-free Aptamer Biosensor with Poly(thionine) as an Electrochemical Probe[J].Chemical Research In Chinese Universities,2013,34(3):551-555. |
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| Authors: | YANG Shao-Ming ZHA Wen-Ling LI Hong SUN Qing LIU Bin ZHENG Long-Zhen |
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| Institution: | Department of Chemistry and Chemical Engineering, School of Basic Sciences, East China Jiaotong University, Nanchang 330013, China |
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| Abstract: | A lable-free aptamer biosensor was fabricated with the electropolymerized poly(thionine) as a redox electrochemical probe and gold nanoparticles as the immobilization matrix for aptamer. Electrochemical impedance spectroscopy was used to monitor the self-assembly process of the aptamer biosensor. The electrochemical behavior of the aptamer biosensor was studied by the cyclic voltammetry(CV) and differential pulse voltammetry(DPV). The biosensor shows a good linear range of 1.0 pg/mL—500 ng/mL for thrombin, R=0.998, with a detection limit of 0.38 pg/mL. The biosensor has simple preparation procedure, high sensitivity and strong anti-interference ability. |
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| Keywords: | Poly(thionine) Gold nanoparticles Aptamer Thrombin Biosensor |
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