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Compact fluorescence depletion microscope system using an integrated optical element |
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| Authors: | Ná ndor Bokor,Yoshinori Iketaki,Masaaki Fujii |
| Affiliation: | a Budapest University of Technology and Economics, Department of Physics, Budafoki u. 8., 1111 Budapest, Hungary b Olympus Corporation, 2-3 Kuboyama-cho, Hachioji-shi 192-8512, Japan c Tokyo Institute of Technology, Chemical Resources Laboratory, 4259 Nagatsuta-cho, Midori-ku, Yokohama 226-8503, Japan |
| Abstract: | The performance of a fluorescence depletion microscope system depends critically on the precise alignment of both the pump and the erase beams with the axis of the focusing objective lens. In this article we propose a technique with which the need for separate alignment of the pump and erase beams is eliminated. Besides being insensitive to alignment errors, our technique is much more compact than present systems, and can serve as a basis for a commercial fluorescence depletion microscope. |
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