| 纳米壳寡糖-铁配合物的制备及其生物活性的研究 |
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| 引用本文： | 黄进,汪世龙,孙晓宇,张蕤,姚思德.纳米壳寡糖-铁配合物的制备及其生物活性的研究[J].化学学报,2006,64(15):1570-1574. |
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| 作者姓名： | 黄进 汪世龙 孙晓宇 张蕤 姚思德 |
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| 作者单位： | (同济大学生命科学与技术学院 上海 200092) |
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| 基金项目： | 国家自然科学基金;上海市纳米科技专项基金 |
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| 摘 要： | 用分子量为5000的壳寡糖与FeCl3反应制得纳米壳寡糖-铁配合物, 并通过FTIR, UV, DTA, TEM, PCS等手段对其结构与形态进行了表征. 同时用紫外光谱法和凝胶电泳法对纳米壳寡糖以及纳米壳寡糖-铁配合物的生物活性进行了研究, 实验结果表明纳米壳寡糖-铁配合物是以静电嵌插的方式与质粒DNA进行结合, 而致使DNA损伤.
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| 关 键 词： | 纳米壳寡糖 纳米壳寡糖-铁配合物 质粒DNA 紫外光谱法 凝胶电泳法 生物活性 |
| 收稿时间： | 01 25 2006 12:00AM |
| 修稿时间： | 2006-01-252006-04-10 |
Study on the Preparation and the Biological Activity of Nano Chitosan-oligosaccharide/Fe Complex |
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| HUANG,Jin,WANG,Shi-Long,SUN,Xiao-Yu,ZHANG,Rui,YAO,Si-De.Study on the Preparation and the Biological Activity of Nano Chitosan-oligosaccharide/Fe Complex[J].Acta Chimica Sinica,2006,64(15):1570-1574. |
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| Authors: | HUANG Jin WANG Shi-Long SUN Xiao-Yu ZHANG Rui YAO Si-De |
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| Institution: | (School of Life Science and Technology, Tongji University, Shanghai 200092) |
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| Abstract: | The nano chitosan-oligosaccharide/Fe complex was prepared by addition of FeCl3 to chito- san-oligosaccharide solution, the molecular weight of which was about 5000. The complex was character- ized by FTIR, UV spectra and DTA, TEM and PCS method. It was concluded that the Fe(III) was coordi- nated with the NH2 of chitosan-oligosaccharide. And the ultraviolet spectroscopy analysis and gel electro- phoresis analysis were used to research the biological activity of chitosan-oligosaccharide and chito- san-oligosaccharide/Fe complex via plasmid DNA. The result indicated that the chitosan-oligosaccharide/Fe complex had a strong binding affinity with DNA and the complex could damage and degrade DNA. |
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| Keywords: | chitosan-oligosaccharide chitosan-oligosaccharide/Fe complex plasmid DNA ultraviolet spectroscopy analysis gel electrophoresis analysis biological activity |
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