| RP-HPLC同时测定溪黄草药材中芦丁和槲皮素的含量 |
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| 引用本文： | 向海艳,谢扬.RP-HPLC同时测定溪黄草药材中芦丁和槲皮素的含量[J].光谱实验室,2011,28(3):1170-1173. |
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| 作者姓名： | 向海艳 谢扬 |
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| 作者单位： | 南方医科大学药学院,广州市沙太南路1023号,510515 |
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| 基金项目： | 广东省教育部产学研合作项目(2009B090300216) |
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| 摘 要： | 采用Kromasil C<,18>柱(250mm×4.6mm,5μm),流动相为甲醇-0.2%磷酸水溶液(45:55,V/V),流速1.0mL/min,检测波长350nm,柱温25℃.结果表明:芦丁和槲皮素进样浓度分别在7.5-240.0μg/mL(r=0.9998)和5.0-160.0μg/mL(r=0.9995)范...
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| 关 键 词： | 溪黄草 反相-高效液相色谱法 芦丁 槲皮素 |
Simultaneous Determination of Rutin and Quercetin in Rabdosia Lophanthoides(Buch.-Ham.ex D.Don)Hara by RP-HPLC |
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| XIANG Hai-Yan XIE Yang.Simultaneous Determination of Rutin and Quercetin in Rabdosia Lophanthoides(Buch.-Ham.ex D.Don)Hara by RP-HPLC[J].Chinese Journal of Spectroscopy Laboratory,2011,28(3):1170-1173. |
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| Authors: | XIANG Hai-Yan XIE Yang |
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| Institution: | XIANG Hai-Yan XIE Yang(College of Pharmaceutical Sciences,Southern Medical University,Guangzhou 510515,P.R.China) |
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| Abstract: | The amounts of rutin and quercetin were detected by HPLC on Kromasil C18 column（250mm×4.6mm,5μm） with methanol-0.2% phosphoric acid（45∶55,V/V）as the mobile phase at flow rate of 1.0mL/min,detection wavelength of 350nm,and column temperature of 25 ℃.The linear ranges were 7.5—240.0μg/mL（r=0.9998） for rutin and 5.0—160.0μg/mL（r=0.9995） for quercetin.The average recoveries（n=3） for rutin and quercetin were 98.78% and 102.05%,respectively.The method is rapid,accurate with good repeatability,and can be applied to control the quality of Rabdosia lophanthoides（Buch.-Ham.ex D.Don） Hara. |
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| Keywords: | Rabdosia lophanthoides(Buch -Ham ex D Don) Hara RP-HPLC Rutin Quercetin |
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